THP-1
GFP plasmid electroporation
mRNA plasmid electroporation
Plasmid electroporation causes cell death in macrophage related cell lines such as THP-1 and U937. Use low centration of plasmid (0.2ug/20ul) helps cell survival. mRNA electroporation gives nearly 100% efficiency yet does not cause cell death in these cells.
THP-1 in 20ul tubes
Cell Number：1-2*10^6 cells
Plasmid：2ug
E buffer：Celetrix buffer
Suggested parameter：Cell Line Mode，500V-540V
Culture medium: 1640+10%FBS (antibiotics free)
THP-1 in 100ul tubes
Cell Number：5-10*10^6 cells
Plasmid：10ug
E buffer：Celetrix buffer
Suggested parameter：Cell Line Mode，500V-540V
Culture medium: 1640+10%FBS (antibiotics free)