Human primary Dendritic cells
Dendritic cells from PBMC, cultured for 6 days with GM-CSF and IL-4 before electroporation.
The DC cells are easy to transfect with the Celetrix electroporation system. Follow the suggested parameters and a high transfection efficiency would be achieved.
A special notice: DC cells have morphology variability among different donors. Cultured in GM-CSF and IL-4 for several days, sometimes they become very adherent to the plate and sometimes they are more like suspension cells. If they adhere to the plate tightly, trypsinize them down with care and try not to allow them sticking to each other and forming big clumps.
Human primary Dendritic cells
in 20ul tubes
Cell Number：1-2*10^6 cells
Plasmid：2ug
E buffer：Celetrix buffer
Suggested parameter：Cell Line Mode，480V-520V
Culture medium: 1640+10%FBS (antibiotics free)
Human primary Dendritic cells
in 100ul tubes
Cell Number：5-10*10^6 cells
Plasmid：10ug
E buffer：Celetrix buffer
Suggested parameter：Cell Line Mode，480V-520V
Culture medium: 1640+10%FBS (antibiotics free)