Human T cells have been hard to transfect with other methods, especially for plasmids.
With Celetrix electroporation, T cells can be transfected any time from the fresh PBMC stage to the stimulated or cultured stage. The Celetrix technology can achieve high transfection efficiency while maintaining high level of cell survival and expansion, allowing immunotherapy applications such as CAR, TCR-T generation and CRISPR knock-down of T cell genes.
Human PBMC CAR expression at 65.9% after 14 days.
Cells form clusters and proliferate similar to un-electroporated cells.
Electroporation |
Viral vectors |
|
Efficiency | High | Low |
Cost | Low | Very High |
Time | Short | Long |
Side effects | Simple | Gene insertion problem |
Celetrix LLC
Address:11100 Endeavor Ct 123 Manassas, VA 20109 US
Phone: 1-646-801-1881
Email: info@celetrix.com